Photocleavage fluorescent labeling compound and application

Abstract: The invention provides a photocleavage fluorescent labeling reversible terminal compound and application of the compound in DNA or RNA sequencing. Photocleavage groups are adopted as inhibition groups, and the inhibition groups are disconnected from basic groups under irradiation of a certain wavelength. The separation process does not need assistance of special catalysts or reduction agents and the like, side reactions of auxiliary reagents, DNA polymerase and the basic groups do not exist, and accordingly reversible termination can be achieved under mild conditions. Besides, the separation process has no interference with the sequencing process, and long-fragment single-molecule sequencing is benefited.
Patent Number: CN106188197(A)
Public Date:
Inventor: GE LIANGJIN; ZHAO LUYANG; XU GUOWEI
Applicant: SHENZHEN HANHAI GENE BIOTECHNOLOGY CO LTD
Application Number: CN201610556330 20160714Global Dossier
Application Date:
Priority Number: CN201510411725 20150714
International Classification: C07H1/00; C07H1/04; C07H19/10; C07H19/20; C09K11/06; C12Q1/68
Cooperative Classification: C07H1/00; C07H1/04; C07H19/10; C07H19/20; C09K11/06; C12Q1/6874; C09K2211/1029; C09K2211/1044; C09K2
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