Recombinant vector and non-GMO gene editing plant screening method

Abstract: The invention discloses a recombinant vector and a non-GMO gene editing plant screening method. An initial carrier in the recombinant vector is a CRISPR/Cas9 carrier which contains sgRNA gene, Cas9 gene and screening label gene and is used for plant gene editing, the recombinant vector carries a BelRNAi expression element, and a hairpin RNA interference fragment interfering with bentazone resistance gene is generated through transcription of the BelRNAi expression element. By introducing the BelRNAi expression element to the initial carrier and conducting bentazone herbicide screening on future generation plants, offspring with target gene mutated are reserved, and it is also ensured that the mutated offspring do not contain transgenic fragments; screening method is cheaper, simpler and more effective.
Patent Number: CN106222193(A)
Public Date:
Inventor: SHU QINGYAO; LU HAIPING; DENG LI; LIU SONGMEI
Applicant: UNIV ZHEJIANG; ZHEJIANG ZHIJIANG SEEDS CO LTD
Application Number: CN20161597205 20160726Global Dossier
Application Date:
Priority Number: CN20161597205 20160726
International Classification: A01H5/00; C12N15/65; C12N15/82
Cooperative Classification: C12N15/65; C12N15/82; C12N15/8209; C12N15/8218; C12N2800/30moredefault
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