Citrus tristeza virus and citrus exocortis viroid dual detection kit and application thereof

Abstract: The invention discloses a citrus tristeza virus and citrus exocortis viroid dual detection kit and application thereof, and designs a dual digital PCR quantitative detection reagent composition for citrus tristeza viruses and citrus exocortis viroids. The kit contains a virus sample RNA extraction reagent, a cDNA reverse transcription synthesis reagent, a digital PCR reagent, a citrus decline and citrus exocortis positive control sample template and a citrus decline and citrus exocortis negative control sample template. The kit has the advantages of high specificity, higher impurity interference resistance, convenience, high speed, detection sensitivity equivalent to that of an existing real-time fluorescent quantitative PCR detection method and the like when being used for detecting the citrus tristeza viruses and the citrus exocortis viroids, and can be used for early qualitative and quantitative detection of the citrus tristeza viruses and the citrus exocortis viroids, epidemiological survey and the like.
Patent Number: CN106244732(A)
Public Date:
Inventor: CHENG BAOPING; PENG AITIAN; ZHAO HONGWEI; SONG XIAOBING; LING JINFENG; CHEN XIA
Applicant: PLANT PROT RES INST GUANGDONG ACAD OF AGRICULTURAL SCIENCES
Application Number: CN20161963212 20161028Global Dossier
Application Date:
Priority Number: CN20161963212 20161028
International Classification: C12N15/11; C12Q1/68; C12Q1/70
Cooperative Classification: C12Q1/6851; C12Q1/701; C12Q2600/16moredefault
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